3MIZ
Crystal structure of a putative transcriptional regulator protein, Lacl family from Rhizobium etli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.508, 71.912, 212.375 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.220 - 1.910 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.27600 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.220 | 1.980 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.096 | 0.329 |
Number of reflections | 41279 | |
<I/σ(I)> | 12 | 2.4 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 13.8 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2M Sodium formate, 20% Polyethylene glycol 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |