3MIX

Crystal structure of the cytosolic domain of B. subtilis FlhA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9330
Spacegroup name	P 65
Unit cell lengths	84.177, 84.177, 118.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.830 - 2.300
R-factor	0.21906
Rwork	0.216
R-free	0.26925
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.288
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.215
Number of reflections	20948
<I/σ(I)>	6.9	5.4
Completeness [%]	99.2	99.2
Redundancy	4.1	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Bis-Tris, pH 6.5, 25 % w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Bis-Tris, pH 6.5, 25 % w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K