3MIT
Structure of Banana lectin-alpha-D-mannose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.926, 80.926, 148.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.460 - 2.320 |
| R-factor | 0.20541 |
| Rwork | 0.203 |
| R-free | 0.24628 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.077 | 0.489 |
| Number of reflections | 24663 | |
| <I/σ(I)> | 16.4 | 3.3 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | ZINC ACETATE DIHYDRATE, SODIUM CACODYLATE, 1,6-HEXANEDIOL, , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
