3MI7
An Enhanced Repressor of Human Papillomavirus E2 Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-31 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.860, 43.099, 45.666 |
| Unit cell angles | 90.00, 120.53, 90.00 |
Refinement procedure
| Resolution | 33.070 - 2.200 |
| R-factor | 0.1878 |
| Rwork | 0.184 |
| R-free | 0.28054 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1by9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.473 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.900 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 4793 | |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 95.4 | 93.1 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 100 mM sodium citrate, 100 mM ammonium acetate, 10% PEG 4000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
