3MHX

Crystal Structure of Stenotrophomonas maltophilia FeoA complexed with Zinc: A Unique Procaryotic SH3 Domain Protein Possibly Acting as a Bacterial Ferrous Iron Transport Activating Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97962
Spacegroup name	P 21 21 21
Unit cell lengths	46.015, 54.031, 61.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.430 - 1.700
Rwork	0.191
R-free	0.22800
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Number of reflections	17106
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	25% v/v PEG 550 MME, 0.1M MES, 0.01M zinc sulfate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K