3MHV
Crystal Structure of Vps4 and Vta1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2009-03-22 |
| Wavelength(s) | 0.9719 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 37.979, 70.322, 88.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.980 - 3.100 |
| R-factor | 0.311 |
| Rwork | 0.298 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries: 3EIE and 2RKL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 3803 | |
| <I/σ(I)> | 19.2 | 2.6 |
| Completeness [%] | 80.0 | 62.1 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 288 | 16%-24% PEG3350, 0.1-0.3M sodium formate, VAPOR DIFFUSION, temperature 288K |
