3MHT

TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH UNMODIFIED DNA AND ADOHCY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	99.860, 99.860, 325.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.189
Rwork	0.189
RMSD bond length	0.030 *
RMSD bond angle	30.100 *
Data reduction software	DENZO
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Rmerge	0.071
Completeness [%]	88.7
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium salfate	1.3-1.7 (M)
2	1	1	sodium citrate	50 (mM)
3	1	2	ammonium salfate	1.3-1.7 (M)
4	1	2	sodium citrate	50 (mM)