3MHP
FNR-recruitment to the thylakoid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.463, 48.824, 71.349 |
| Unit cell angles | 106.56, 97.01, 91.77 |
Refinement procedure
| Resolution | 14.980 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qg0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.035 | 0.236 |
| Number of reflections | 66902 | |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 10.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 200 mM Ammoniumcitrate 20 % PEG 3350 crystal growth - 3 month, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
