3MHA
Crystal structure of LprG from Mycobacterium tuberculosis bound to PIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.158, 71.254, 61.330 |
| Unit cell angles | 90.00, 106.16, 90.00 |
Refinement procedure
| Resolution | 32.047 - 1.850 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mh8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.144 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.730 | 1.850 |
| Rmerge | 0.052 | 0.624 |
| Number of reflections | 35778 | |
| <I/σ(I)> | 4.7 | 5.5 |
| Completeness [%] | 86.6 | 82.2 |
| Redundancy | 5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 100mM Sodium acetate trihydrate, 25% PEG3350, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
