3MH8
Crystal structure of LprG from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.296, 71.887, 61.266 |
| Unit cell angles | 90.00, 106.32, 90.00 |
Refinement procedure
| Resolution | 18.921 - 1.995 |
| R-factor | 0.2315 |
| Rwork | 0.229 |
| R-free | 0.27790 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.995 | 2.000 |
| Rmerge | 0.070 | 0.599 |
| Number of reflections | 26692 | |
| <I/σ(I)> | 461.1 | 13.7 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 100mM Sodium acetate trihydrate, 25% PEG3350, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
