3MH7
HtrA proteases are activated by a conserved mechanism that can be triggered by distinct molecular cues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 253.929, 253.929, 253.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.321 - 2.961 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.190 |
| High resolution limit [Å] | 2.961 | 2.961 |
| Rmerge | 0.093 | 0.579 |
| Number of reflections | 15145 | |
| <I/σ(I)> | 13 | 2.9 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 292 | PEG 550 MME, NaCl, pH 8.5, VAPOR DIFFUSION, temperature 292K |
