3MH3
Mutagenesis of p38 MAP kinase establishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-out state
Replaces: 2PTJExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2004-08-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.520, 73.850, 76.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.990 - 2.200 |
R-factor | 0.23728 |
Rwork | 0.233 |
R-free | 0.29322 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyj |
RMSD bond length | 0.020 |
RMSD bond angle | 1.783 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.370 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | 0.441 |
Number of reflections | 19105 | |
<I/σ(I)> | 7.1 | 2 |
Completeness [%] | 96.7 | 96.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 10-20% PEG 4000, 0.1 M cacodylic acid, 50 mM n-octyl-beta-D-glucoside, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |