3MGX
Crystal Structure of P450 OxyD that is involved in the Biosynthesis of Vancomycin-type Antibiotics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.850, 61.050, 100.640 |
| Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.100 |
| R-factor | 0.20913 |
| Rwork | 0.208 |
| R-free | 0.23302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ejb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 44661 | |
| <I/σ(I)> | 19.2 | 4.5 |
| Completeness [%] | 97.5 | 92.4 |
| Redundancy | 7.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 18% PEG 2000 MME, 0.1M Bis-Tris, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
