3MGC
Teg12 Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.388, 126.086, 145.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.570 - 2.910 |
| R-factor | 0.21957 |
| Rwork | 0.217 |
| R-free | 0.27193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ov8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.495 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.570 | 3.030 |
| High resolution limit [Å] | 2.910 | 2.930 |
| Rmerge | 0.063 | 0.396 |
| Number of reflections | 14643 | |
| <I/σ(I)> | 19.1 | 2.03 |
| Completeness [%] | 95.5 | 98 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | Protein concentration of 7.5 mg/ml. 1:1 ul ratio of protein to crystallization solution. 1.0 M Sodium Citrate, 0.1 M Sodium Cacodylate, pH 6.5, Silver Bullet Reagent 29 (Hampton), VAPOR DIFFUSION, temperature 295K |
