3MG8
Structure of yeast 20S open-gate proteasome with Compound 16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.333, 299.185, 145.667 |
| Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
| Resolution | 49.880 - 2.590 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 5.600 | 2.590 |
| Rmerge | 0.073 | 0.036 | 0.327 |
| Number of reflections | 315407 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 95.6 | 93.4 | 85.9 |
| Redundancy | 3.3 | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
