3MG3

Crystal structure of the orange carotenoid protein R155L mutant from cyanobacteria synechocystis sp. PCC 6803

Replaces:  3I1X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 32
Unit cell lengths	82.683, 82.683, 86.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.341 - 1.702
R-factor	0.1552
Rwork	0.153
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I1V
RMSD bond length	0.014
RMSD bond angle	1.432
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.072	0.044	0.551
Number of reflections	71874
<I/σ(I)>	15.2
Completeness [%]	99.0	99.8	98
Redundancy	5.7	5.9	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	12% PEG 3350, 100mM Sodium acetate, 2% Glycerol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K