3MFQ
A Glance into the Metal Binding Specificity of TroA: Where Elaborate Behaviors Occur in the Active Center
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 102.360, 102.360, 107.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.443 - 2.598 |
| R-factor | 0.232 |
| Rwork | 0.226 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0f |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.598 | 2.598 |
| Rmerge | 0.085 | 0.085 |
| Number of reflections | 30968 | |
| Completeness [%] | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 2.1M Ammonium Sulfate, 6%(v/v) iso-propanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
