3MFN
Dfer_2879 protein of unknown function from Dyadobacter fermentans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.779, 83.650, 93.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.300 - 2.020 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.462 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.092 | 0.846 |
| Number of reflections | 36244 | |
| <I/σ(I)> | 9 | 2.67 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 14 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.2 M sodium acetate, 0.2 M ammonium acetate, 30% PEG-4000, 1/70 chymotrypsin, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
