3MFK
Ets1 complex with stromelysin-1 promoter DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.812, 99.153, 76.496 |
| Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
| Resolution | 36.350 - 3.000 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gvj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.438 |
| Number of reflections | 13212 | |
| <I/σ(I)> | 29.9 | 4.46 |
| Completeness [%] | 97.9 | 96.1 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 15 mM ammonium chloride, 15 mM magnesium chloride, 0.7 M NDSB-195, 40 mM MES buffer (pH 6.5) and 11.5% v/v 2-Propanol, VAPOR DIFFUSION, SITTING DROP, temperature 295 K |
