3MFB
Crystal Structure of the S-type Pyocin domain of ECA1669 protein from Erwinia carotovora, Northeast Structural Genomics Consortium Target EwR82C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97885 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.076, 52.307, 62.161 |
| Unit cell angles | 69.50, 90.04, 90.04 |
Refinement procedure
| Resolution | 19.410 - 2.200 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.104 |
| Number of reflections | 56618 | |
| <I/σ(I)> | 15.4 | 7.3 |
| Completeness [%] | 93.7 | 83.9 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M MES (pH 6.5), 30% PEG 5K MME, and 0.2M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
