3MEW
Crystal structure of Novel Tudor domain-containing protein SGF29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.623, 41.424, 52.324 |
| Unit cell angles | 90.00, 121.43, 90.00 |
Refinement procedure
| Resolution | 44.650 - 1.920 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3me9 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.650 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.038 | 0.119 |
| Number of reflections | 12062 | |
| <I/σ(I)> | 37.9 | 15.4 |
| Completeness [%] | 92.2 | 88.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 20-28% PEG3350, 0.1M Bis-Tris pH 5.5, vapor diffusion, Sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP |
