3MER
Crystal Structure of the methyltransferase Slr1183 from Synechocystis sp. PCC 6803, Northeast Structural Genomics Consortium Target SgR145
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00684 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.440, 53.792, 53.845 |
| Unit cell angles | 115.49, 95.66, 84.35 |
Refinement procedure
| Resolution | 19.610 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i6g and 2kw5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.227 |
| Number of reflections | 20859 | |
| <I/σ(I)> | 23.4 | 4.7 |
| Completeness [%] | 98.1 | 96.3 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch, under oil | 4 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M Sodium Malonate (pH 4.0) and 12% PEG 3350. , Microbatch, under oil, temperature 277K |
