3MEI
Regulatory motif from the thymidylate synthase mRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9197 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.928, 38.564, 41.985 |
| Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
| Resolution | 28.197 - 1.968 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 4.220 | 1.960 |
| Rmerge | 0.064 | 0.054 | 0.154 |
| Number of reflections | 13933 | ||
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 97.8 | 99 | 81.9 |
| Redundancy | 8.5 | 9.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
