3ME9

Crystal structure of SGF29 in complex with H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97927
Spacegroup name	P 21 21 21
Unit cell lengths	50.038, 65.434, 105.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.370
R-factor	0.19167
Rwork	0.191
R-free	0.21353
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.563
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.390
High resolution limit [Å]	1.370	3.710	1.370
Rmerge	0.080	0.041	0.972
Number of reflections	73960
<I/σ(I)>	6.7
Completeness [%]	100.0	99.9	100
Redundancy	7.1	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	25% PEG3350, 0.1M ammonium sulfate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, sitting drop, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	25% PEG3350, 0.1M ammonium sulfate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, sitting drop, temperature 291K