3ME1
Crystal Structure of the Desulfovibro vulgaris Urea Transporter in the P3(1) Space Group at 3.86
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 |
| Unit cell lengths | 103.197, 103.197, 141.498 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.813 - 3.862 |
| R-factor | 0.3239 |
| Rwork | 0.321 |
| R-free | 0.38660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.945 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.920 |
| High resolution limit [Å] | 3.850 | 10.420 | 3.850 |
| Rmerge | 0.067 | 0.032 | 0.582 |
| Number of reflections | 15450 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 97.7 | 90.9 | 81.4 |
| Redundancy | 10.7 | 10.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 22% PEG 1500, 100 mM Na Cacodylate, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
