3ME0

Structure of the E. coli chaperone PAPD in complex with the pilin domain of the PapGII adhesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-17
Detector	NOIR-1
Spacegroup name	P 21 21 2
Unit cell lengths	103.665, 65.845, 56.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.260 - 2.030
R-factor	0.182
Rwork	0.182
R-free	0.19000
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.114
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER (EP)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.400	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.108
Number of reflections	24169
<I/σ(I)>	23.9	5.7
Completeness [%]	99.9	97.7
Redundancy	14	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	1M SODIUM MALONATE, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K