3MDN
Structure of glutamine aminotransferase class-II domain protein (SPO2029) from silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.367, 72.960, 106.722 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.090 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.461 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 5.700 | 2.100 |
| Rmerge | 0.063 | 0.049 | 0.690 |
| Number of reflections | 59569 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 99.8 | 97.8 | 100 |
| Redundancy | 6.1 | 5.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M MgCl2, 25% PEG 3350, pH 7.5, Vapor diffusion, Sitting drop, temperature 298K |
