3MDA
DNA polymerase lambda in complex with araC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-14 |
| Detector | RIGAKU |
| Wavelength(s) | 1.504 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.068, 62.637, 140.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.565 - 2.031 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 52786 |
| Completeness [%] | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 5.5 | 277 | 2-propanol, pH 5.5, hanging drop, temperature 277K |
