3MD9
Structure of apo form of a periplasmic heme binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | PIXEL |
| Collection date | 2009-10-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.710, 66.740, 62.270 |
| Unit cell angles | 90.00, 110.70, 90.00 |
Refinement procedure
| Resolution | 29.125 - 1.500 |
| R-factor | 0.1541 |
| Rwork | 0.152 |
| R-free | 0.18120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3MD8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.130 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.089 | 0.528 |
| Number of reflections | 78325 | |
| <I/σ(I)> | 14.24 | 4.31 |
| Completeness [%] | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283 | 29% (w/v) PEG 5K MME, 0.1 M Magnesium acetate, 0.1 MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
