3MD5
Prion peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 19.618, 9.459, 19.672 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 19.650 - 1.400 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.432 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
| Rmerge | 0.057 | 0.053 | 0.133 |
| Number of reflections | 1309 | ||
| <I/σ(I)> | 29.3 | ||
| Completeness [%] | 84.1 | 93.6 | 61.7 |
| Redundancy | 10.1 | 13.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.3 M ammonium acetate, 30-40% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
