3MD2
Crystal structure of the matrix protein 1 from influenza A virus (A/California/04/2009 (H1N1))
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-23 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.246, 37.748, 122.221 |
| Unit cell angles | 89.98, 90.00, 112.53 |
Refinement procedure
| Resolution | 34.868 - 2.200 |
| R-factor | 0.2144 |
| Rwork | 0.212 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z16 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.345 |
| Number of reflections | 23637 | |
| <I/σ(I)> | 10.3 | 2.6 |
| Completeness [%] | 96.4 | 84.6 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M Ammonium dihydrogen Phosphate, 20% w/v PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
