3MD0

Crystal structure of arginine/ornithine transport system ATPase from Mycobacterium tuberculosis bound to GDP (a RAS-like GTPase superfamily protein)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-24
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	65.530, 187.360, 66.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.450
R-factor	0.213
Rwork	0.211
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qm7
RMSD bond length	0.015
RMSD bond angle	1.556
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.072	0.573
Number of reflections	15339	1090
<I/σ(I)>	20.5	2.7
Completeness [%]	99.4	97.2
Redundancy	6.84	4.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	289	50% PEG 200, 0.2 M NaCl, 0.1 M Na K phosphate pH 6.2, 22.0 mg/mL protein, 2 mM GDP, crystal tracking ID 207391d3, VAPOR DIFFUSION, SITTING DROP, temperature 289K