3MCZ
The Structure of an O-methyltransferase family protein from Burkholderia thailandensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.252, 136.595, 56.080 |
| Unit cell angles | 90.00, 102.55, 90.00 |
Refinement procedure
| Resolution | 38.620 - 1.900 |
| R-factor | 0.152 |
| Rwork | 0.149 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.388 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | |
| Number of reflections | 47531 | |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
