3MCL
Anti-beta-amyloid antibody c706 fab in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-02-27 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.330, 62.790, 68.600 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.20481 |
| Rwork | 0.203 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eo8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.364 |
| Number of reflections | 42232 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 95.6 | 85.4 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% PEG 3350, 0.2 M SODIUM THIOCYANATE; CRYO CONDITIONS: 0.1 M HEPES PH 7.0, 24% PEG 3350, 0.2 M SODIUM THIOCYANATE, 20% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
