3MCG

THREE-DIMENSIONAL STRUCTURE OF A LIGHT CHAIN DIMER CRYSTALLIZED IN WATER. CONFORMATIONAL FLEXIBILITY OF A MOLECULE IN TWO CRYSTAL FORMS

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	72.800, 81.900, 71.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.208
RMSD bond length	0.026
RMSD bond angle	0.056
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Number of reflections	7273 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *	4 *	taken from Abola, E.E. et al (1980). Biochemistry, 19, 432-439. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	35-40 (mg/ml)
2	1	2	Tris-HCl	0.02 (M)	in 0.001 M in EDTA