3MCE
Crystal structure of the NAC domain of alpha subunit of nascent polypeptide-associated complex(NAC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.592, 59.605, 69.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.901 - 2.396 |
| R-factor | 0.25 |
| Rwork | 0.247 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 1.972 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.396 | 2.400 |
| Rmerge | 0.075 | 0.286 |
| Number of reflections | 9272 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 13.6 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 289 | 0.2M potassium citrate tribasic monohydrate (pH 8.3), 20% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
