3MBV
Structure of bacterirhodopsin crystallized in betta-XylOC(16+4) meso phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.348, 61.348, 109.787 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.830 - 2.000 |
| Rwork | 0.181 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.537 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.130 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.125 | 0.469 |
| Number of reflections | 13746 | |
| <I/σ(I)> | 9.2 | 2.6 |
| Completeness [%] | 87.5 | 87 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | in meso crystallization | 298 | in meso crystallization, temperature 298K |
