3MB5
Crystal structure of P. abyssi tRNA m1A58 methyltransferase in complex with S-adenosyl-L-methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 86.550, 89.260, 110.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.700 - 1.600 |
R-factor | 0.18473 |
Rwork | 0.184 |
R-free | 0.19891 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 3LGA |
RMSD bond length | 0.012 |
RMSD bond angle | 1.373 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.103 | 0.317 |
Number of reflections | 56562 | |
<I/σ(I)> | 16.42 | 5.1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 9.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2.8M ammonium sulfate, 0.2M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |