3MAF
Crystal structure of StSPL (asymmetric form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.190, 84.900, 131.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.665 - 2.971 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dgk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.4_29) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Number of reflections | 19779 | 2205 |
| <I/σ(I)> | 13.7 | 4.3 |
| Completeness [%] | 98.7 | 92.8 |
| Redundancy | 5.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | PEG 2000 MME 25%, 0.2 M MgCl2, 0.1 M Tris(HOAc) pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
