3MAC
crystal structure of GP41-derived protein complexed with fab 8062
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | MARCCD300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.668, 41.741, 98.602 |
| Unit cell angles | 90.00, 94.85, 90.00 |
Refinement procedure
| Resolution | 29.500 - 2.500 |
| R-factor | 0.20163 |
| Rwork | 0.200 |
| R-free | 0.26441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cmr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 23655 | |
| <I/σ(I)> | 13.79 | 2 |
| Completeness [%] | 99.1 | 96.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 14% Polyethylene glycol 10K, 0.1M ammonium sulfate, 5% ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
