3MAB
CRYSTAL STRUCTURE OF AN UNCHARACTERIZED PROTEIN FROM LISTERIA MONOCYTOGENES, Triclinic FORM
Replaces: 3BQSExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.062, 33.549, 40.784 |
| Unit cell angles | 83.24, 75.47, 89.73 |
Refinement procedure
| Resolution | 16.870 - 1.420 |
| R-factor | 0.16883 |
| Rwork | 0.167 |
| R-free | 0.20633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bqt |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.756 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.058 | 0.260 |
| Number of reflections | 50049 | |
| <I/σ(I)> | 11.4 | 4.4 |
| Completeness [%] | 89.0 | 82.5 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.2M ammonium acetate, 0.1m Na citrate, 30% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
