3MAA
Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with Adenosine 5-O-(l-Thiophosphate) and Low Ca Concentration
Replaces: 3E8AExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0231 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.744, 133.722, 70.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| R-factor | 0.24228 |
| Rwork | 0.239 |
| R-free | 0.30888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azs |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.077 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.030 | |
| Number of reflections | 20034 | |
| <I/σ(I)> | 5.1 | 3.3 |
| Completeness [%] | 82.7 | 2.95 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 289 | 7.5-7.8% PEG8000, 0.5M NACL, 0.1M PHOSPHATE BUFFER, pH 5.6, VAPOR DIFFUSION, temperature 289K |
