3MA0
Closed liganded crystal structure of xylose binding protein from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 156.064, 156.064, 103.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.18306 |
| Rwork | 0.182 |
| R-free | 0.20163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ioy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.314 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.400 |
| Number of reflections | 141545 | |
| <I/σ(I)> | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 2.4M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
