3M9Z
Crystal Structure of extracellular domain of mouse NKR-P1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 59.912, 59.912, 159.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.19696 |
| Rwork | 0.195 |
| R-free | 0.23927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e87 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.741 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.704 |
| Number of reflections | 16416 | |
| <I/σ(I)> | 14.3 | 2.1 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.3M Ammonium phosphate, not buffered, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
