3M9W
Open ligand-free crystal structure of xylose binding protein from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.955 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.663, 72.188, 66.322 |
| Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.21032 |
| Rwork | 0.208 |
| R-free | 0.26429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ioy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.223 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.084 | 0.372 |
| Number of reflections | 17127 | |
| <I/σ(I)> | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 21%(w/v) polyethylene glycol 3350, 0.2M ammonium dihydrogen phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
