3M9I
Electron crystallographic structure of lens Aquaporin-0 (AQP0) (lens MIP) in E. coli polar lipids
Experimental procedure
| Source type | ELECTRON MICROSCOPE |
| Source details | OTHER |
| Temperature [K] | 279 |
| Detector technology | CCD |
| Collection date | 2009-02-24 |
| Detector | Gatan |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 65.500, 65.500, 200.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.890 - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b6o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.800 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.890 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.226 | |
| Number of reflections | 14417 | |
| Completeness [%] | 92.3 | 83.7 |
| Redundancy | 8.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6 | 300 | 100 mM NaCl, 50mM MgCl2, 10mM MES pH 6.0, MICRODIALYSIS, temperature 300K |
