3M97
Structure of the soluble domain of cytochrome c552 with its flexible linker segment from Paracoccus denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.376, 38.991, 93.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.130 - 1.332 |
| R-factor | 0.20103 |
| Rwork | 0.200 |
| R-free | 0.22906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ql4 |
| RMSD bond length | 0.037 |
| RMSD bond angle | 3.339 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.130 | |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.110 | 0.110 |
| Number of reflections | 31610 | |
| <I/σ(I)> | 29.79 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20 % PEG 550 MME, 0.1 M Zinc sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
