3M95
Crystal structure of autophagy-related protein Atg8 from the silkworm Bombyx mori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 96.768, 96.768, 44.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.080 - 2.400 |
| R-factor | 0.23329 |
| Rwork | 0.231 |
| R-free | 0.27309 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kjt |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.941 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.700 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.083 | |
| Number of reflections | 14385 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 89.6 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 2.0M Ammonium Sulfate, 0.1M Sodium Citrate, 0.2M Sodium/Potassium Tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
