3M93

Complex crystal structure of Ascaris suum eIF4E-3 with m7G cap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-10
Detector	NOIR-1
Wavelength(s)	1
Spacegroup name	I 41
Unit cell lengths	104.620, 104.620, 47.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.920 - 2.900
R-factor	0.24469
Rwork	0.239
R-free	0.29372
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v8w
RMSD bond length	0.011
RMSD bond angle	1.353
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASES
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.990	2.974
High resolution limit [Å]	2.900	2.900
Rmerge	0.113
Number of reflections	5719
Completeness [%]	98.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	277	20% MMG PEG 2000, 0.2 M (NH4)2SO4, and 100 mM Na-Critate, pH 5.6, VAPOR DIFFUSION, temperature 277K