3M8Z
Phosphopentomutase from Bacillus cereus bound with ribose-5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.588, 76.755, 107.083 |
| Unit cell angles | 90.00, 108.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.19830 |
| Starting model (for MR) | 3m8w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 128719 | |
| <I/σ(I)> | 25.7 | 2.5 |
| Completeness [%] | 98.8 | 90.3 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
